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KMID : 0355420090330040534
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Journal of Korean Academy of Oral Health
2009 Volume.33 No. 4 p.534 ~ p.543
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The Inhibitive effect of 24 months xylitol gum chewing on the virulence related gene expression of Streptococcus mutans
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Kim Ji-Hye
Seo Young-Gyu
Lee Young-Eun
Ahn Sang-Hun
Gu Hyo-Jin
Choi Youn-Hee
Song Keun-Bae
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Abstract
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Objective: The aim of this study was to examine the effect of chewing gum containing xylitol on inhibiting the virulence related gene expression of Streptococcus mutans (S. mutans).
Methods: The subjects voluntarily participating in a women’s oral health prevention program were divided into two groups (a control and a xylitol group), and then they were followed for 2 years. At baseline, 6, 12, 18 and 24 months whole stimulated saliva samples were collected from the subjects. The analysis of the colony count, stereomicroscopy and real-time reverse transcription polymerase chain reaction were performed to analyze the characteristics of S. mutans.
Results: The S. mutans counts decreased steadily in the xylitol group over the study period (p< 0.05). In addition, the size of the colonies isolated from the xylitol group was smaller and their rough surface changed into a smooth surface. The expression of gtfA, gtfB, gtfC, gtfD, ftf, Putative inorganic ion ABC transporter and ATP-binding protein gene were significantly reduced in the xylitol group (p<0.05). The expression of gtfA, gtfB, gtfD, ftf, Putative inorganic ion ABC transporter and the ATP-binding protein gene in the xylitol group were significantly lower than those of the control group at 6, 12, 18 and 24 months (p< 0.05).
Conclusions: These findings indicate that chewing xylitol gum over a long period may decrease the expression of the genes associated with S. mutans virulence, which can result in the reduced size and growth of S. mutans colonies.
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KEYWORD
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Streptococcus mutans, virulence related gene expression, xylitol
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